Pyrogens can trigger inflammatory responses and affect the safety and reliability of pharmaceutical products and research applications. While bacterial endotoxins represent an important group of pyrogens, other pyrogenic substances may require a broader testing approach.
Microcoat combines established testing methods with scientific expertise to support pyrogen detection, investigate challenging samples, and develop testing strategies aligned with product-specific requirements.
The Monocyte Activation Test (MAT) is a human cell-based approach for detecting pyrogenic substances. It measures the cellular response to pyrogenic stimulation and can provide a broader assessment than endotoxin-specific assays.
Microcoat works with MAT approaches based on human immune cells, including peripheral blood mononuclear cells (PBMCs), cryopreserved blood-derived material and cell lines, depending on the selected test system.
Sample is introduced to a suitable human cell-based test system.
Pyrogenic stimulation triggers an immune cell response.
A suitable inflammatory marker is measured and evaluated.
Whole blood cytokine release assays measure the release of pro-inflammatory cytokines after fresh human whole blood has been exposed to a test sample. They complement pyrogen testing and support the immunological characterization of biopharmaceuticals.
Microcoat adapts the assay design to your product and question — from stimulation conditions to the choice of cytokines and readout.
Fresh human whole blood is incubated with the sample under defined conditions.
Immune cells respond with the release of pro-inflammatory cytokines such as IL-1β, IL-6 or TNF-α.
Cytokine levels are quantified and evaluated against suitable controls.
Microcoat offers confidential proficiency testing for laboratories performing the Monocyte Activation Test. Blinded samples allow you to assess the performance of your test system independently and document it for quality assurance.
Results are evaluated confidentially and reported in a way that supports internal reviews, audits and continuous improvement.
Participants receive characterized samples with unknown pyrogen content.
Samples are analyzed with the laboratory's own MAT procedure.
Results are evaluated and reported confidentially for each participant.
Interference, unexpected results or difficult matrices can make pyrogen testing challenging. Microcoat supports the investigation of such issues and helps establish robust, product-specific testing strategies.
Our scientists combine practical MAT experience with an understanding of regulatory requirements to find workable solutions.
Identify potential causes of interference or unexpected results.
Evaluate sample preparation, dilution and alternative test conditions.
Support method suitability, validation and the interpretation of results.
Tell us about your sample, testing requirements, cytokine release assessment or MAT proficiency evaluation. Our scientific team will review your inquiry and discuss suitable next steps.
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Share a few details about your testing requirements. Our team will get in touch to discuss your project.
For the detection and investigation of bacterial endotoxins, Microcoat offers dedicated endotoxin testing services, including LAL, recombinant Factor C, EndoLISA and Low Endotoxin Recovery (LER) studies.
From assay development to commercial manufacturing — we support your project at every stage.
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