Assay Development explained
In short
Assay development is the process of turning a measurement idea into a reliable test. It runs from feasibility and design, through optimisation of reagents and conditions, to validation that proves the assay is accurate, precise and robust.
Development usually begins with a feasibility phase that confirms the target can be measured with acceptable signal and specificity. Promising conditions then move into systematic optimisation.
Optimisation tunes antibodies, buffers, incubation and detection to maximise robustness. Validation then demonstrates accuracy, precision, linearity and stability against predefined acceptance criteria.
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