Solid phase coating

Professional coating of all diagnostic devices and particles

Diagnostic kit manufacturers require procedures ensuring high process safety and security of supply, using raw materials of excellent quality and consistency. Rely on Microcoat for your diagnostic manufacturing needs. High quality solid supports coated by Microcoat are one basic ingredient for a superior quality of diagnostic kits. We recommend using our streptavidin-biotin technology for high signals, low intra and inter plate variance and best lot-to-lot reproducibility if suitable to your assay principle.

Our Know-how

We focus on implementing a robust and cost-effective manufacturing process in order to ensure
longtime secure product supply.

Technologies

Direct adsorptive coating of antibodies and proteins

Suitable for microplates and gold surfaces (including colloidal gold), optimization necessary for each target protein.

Covalent coupling of proteins, antibodies, peptides, nucleic acids

Suitable for microplates (pretreatment for activation necessary), magnetic beads, latex particles

Streptavidin-coating

Suitable for all kind of surfaces including microplates, magnetic particles, gold surfaces, latex particles, foils.
Usually yielding in a very stable product that can easily combined in a second step with any biotinylated compound.

Microcoat provides high quality solid phase coating services including

Streptavidin-based or direct coating

The microplate format is the world standard for analytical testing. Coated microplates are used in the fields of immune diagnostics, molecular diagnostics and high-throughput screening. Microcoat offers proven coating strategies for proteins, nucleic acids or small molecules with established IPC and QC procedures.

Using professional manufacturing equipment, we coat microplates e.g. for ELISA and ELISpot applications of all formats and from all suppliers.

Processes

Coating of 96-well and 384-well plates
Automation for plate and tube coating
Maximum flexibility regarding plate design and packaging
Production in cleanroom environment
Lot sizes range from 100 to 10,000 plates or more
Special stabilization know-how
More than 60 different coating variants

Being a B2B manufacturer Microcoat is producing make-to-order according to customer specifications.
Lot size of one batch can be up to 10,000 plates or even more.
Smaller quantities of two streptavidin microplate variants specified below are available from stock.

DOWNLOADS

Magnetic particles - streptavidin magnetic beads

Beads are widely used for sample preparation or as a direct label. Microcoat functionalizes beads of any kind, independent on size or chemical nature. Different coating strategies are available: adsorptive coating, covalent linking or the universal streptavidin-biotin system.

Magnetic separation is highly efficient for purification and enrichment of analytes from complex mixtures or as solid phase in enzymatic reactions and immunoassays.

Formats

Immuno-separation
Nucleic acid purification
Bead-based immunoassays
Isolation of bacteria

Besides the universal streptavidin-beads alternative functionalization methods can be offered (adsorptiv or covalent coating).

DOWNLOADS

Colloidal gold (streptavidin-based or direct coating)

Colloidal gold is a very sensitive direct label used in rapid tests (lateral flow technology). Due to the small size, gold nanoparticles appear intensely red and remain in suspension. In principal, any kind of molecule can be conjugated to gold particles.

Colloidal gold - Colloidal gold conjugation

Colloidal gold is a suspension of gold nanoparticles with intensely red color. It is used as a sensitive direct label for rapid diagnostic tests (test strips). For this application the gold particles must be conjugated to the specific protein component.

Methods

Direct conjugation of proteins to colloidal gold
Binding of biotinylated proteins to universal streptavidin gold
Streptavidin gold - available from stock

DOWNLOADS

Customized formats

Based on the established know-how, Microcoat develops coating procedures for films, slides, membranes or sensor surfaces. We partner system development projects and offer development support for innovative diagnostic platforms.

For specialized diagnostic applications, new innovative test concepts are required. On the basis of customer specifications Microcoat develops coating procedures and manufacturing processes for:

custom

Methods

Tubes
Beads
Membranes
Foils
Arrays
Test strips
Gold surfaces
Sensor surfaces
Microfluidic devices

Besides the universal streptavidin-biotin technology,

alternative functionalization methods are available (adsorptiv or covalent coating).

Inert materials can be activated by chemical or physical methods prior to coating.

Complementary services:

Optimal coating strategy

based on the selection of specific and robust conjugates of your antibodies and proteins

Optimal coating strategy

based on the selection of specific and robust conjugates of your antibodies and proteins

Assay development

Building the perfect assay for your application based on indivdually optimized reagents

Assay development

Building the perfect assay for your application based on indivdually optimized reagents

Benchmarking studies

As a third party service provider we compare your assay to available products on the market

Benchmarking studies

As a third party service provider we compare your assay to available products on the market