Lateral Flow Rapid Prototyping Platform

From feasibility to final product

Microcoat offers an unique universal lateral flow rapid prototyping platform for fast and cost effective feasibility studies. With this platform it is possible to overcome the various obstacles of a long and often tedious lateral flow test development: e.g. material screening, development of stable detection particle conjugates and their reliable immobilization, polyhapten development and immobilization on the test strip and selection of a suitable control line system. Microcoat’s rapid prototyping platform is based on a ready-to-use lateral flow strip compatible with any sandwich detection system.

Lateral flow rapid prototyping platform:

Fast and cost-effective feasibility studies, stable reagents, reliable production

 

Microcoat’s rapid prototyping lateral flow strip

Microcoat’s rapid prototyping lateral flow strip

Performance of the rapid prototyping lateral flow strip

Applicable for various - even complex - matrices (e.g. serum, aqueous extracts of food or environmental samples)
Typical detection limit in the low ng/mL (ppb) range
Usual testing time 3 - 10 minutes
Suitable sample volume: 150 - 500 μL

Benefits

Microcoat’s rapid prototyping platform offers great opportunities for many tasks:

Short and cost-effective proof of principle
Simple transfer of a classical ELISA to a Lateral Flow Test
Direct as well as competitive test format feasible (depending on analyte)
Additional services: development of kit concept and routine production
Simple dipstick format or assembled in cassettes

Rapid Prototyping Project Phases

Rapid Prototyping Project Phases

Idea

Helping you with the details of your lateral flow test idea

Determination of crucial corner points of your lateral flow idea by our team

Feasibility study

Milestone-driven project execution

Reliable conjugation of antibody/antibodies with specific labels appropriate for our rapid prototyping platform

Evaluation of basic test parameters, e.g. antibody concentration and ratio, specificity and sensitivity, limit of detection (LOD) and quantification (LOQ), cross reactivity

Lateral flow test prototype

Determination of specifications according to the results of the feasibility study

Production of your lateral flow test prototype

Development

Support during the decision for a kit concept including all kit components required, e.g. lateral flow strips, antibodies, buffer, labels, packaging, additional materials like pipettes, reaction tubes, swabs

Determination of a suitable antibody conjugate preparation for your kit concept (e.g. liquid in dropper bottles, lyophilized or dried in tubes)

Process and product verification including stability studies

Consulting regarding regulatory aspects (e.g. IVD)

Smooth transfer to routine production

Market-ready lateral flow test kit

Production of your lateral flow test kit in flexible batch sizes according to your requirements

Practical example

Lateral flow prototype for analysis of inflammatory marker IL-6 in human serum

Sample: 150 μL human serum + 50 μL buffer

Assay time: 10 min (5 min pre-incubation, 5 min test strip run)

LOD: 2 ng/mL IL-6 in human serum

LOQ: 5 ng/mL IL-6 in human serum

Linear range: 0 - 50 ng/mL

DOWNLOADS

If you are interested in our rapid prototyping platform please download and fill-in the contact form below.

Product Brochure
Product Brochure
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Questionnaire
Questionnaire
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Have a look:

From idea to product

Microcoat develops and optimizes your test assay concept into market-ready kits

From idea to product

Microcoat develops and optimizes your test assay concept into market-ready kits

For ELISpot application...

... we produce coated microwell plates in flexible plate design and packaging

For ELISpot application...

... we produce coated microwell plates in flexible plate design and packaging

Did you know?

In our certified GLP test facility we offer bioanalytical services for clinical studies phase I - III

Did you know?

In our certified GLP test facility we offer bioanalytical services for clinical studies phase I - III