Assay development

Optimization and validation services

Specific diagnostic tests are required for pre-clinical and clinical phases of drug development as well as for patient stratification (companion diagnostics, personalized medicine). For protein therapeutics immunoassays are the methods of choice, monitoring clearance (pharmacokinetic) and assessing immunogenicity. In other fields such as veterinary, agricultural and environmental, biomarkers are more frequently used as decisive parameters prior to treatment.

Our Cornerstones

A broad spectrum of methods, long-lasting experience and intensive communication with the customer are the cornerstones for successful project execution.

Immunoassays

Microcoat offers a complete solution for your specific immunoassay. Assay formats comprise.

Sandwich, competitive and bridging ELISA in 96- and 384-well plates
Lateral flow tests strips and line blots
Bead-based assays (magnetic separation, latex agglutination)
Cell-based assays
ELISpot assays
Customer specific assay platforms

Molecular assays

Development of qualitative and quantitative PCR assays including sample preparation.

Sample stabilization
Nuleic acid isolation
Primer / probe design
Assay development
Assay validation
Production of components and kits

Cell-based assays

Microcoat’s cell biology laboratory provides infrastructure for the cultivation of cell lines and primary cells.
Focus is the establishment of cell systems for production (lab-scale) and development of cell-based assays.

Isolation and cultivation of primary cells
Cell systems for transfection and protein expression
Test systems for neutralizing anti-drug antibodies (NADA)
Monocyte activation tests

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Critical reagent supply

Microcoat offers the modification and conjugation of antibodies, proteins and antigens for your individual assay

Critical reagent supply

Microcoat offers the modification and conjugation of antibodies, proteins and antigens for your individual assay

product realization

We develop your assay into a lab kit, prototype or market-ready product

product realization

We develop your assay into a lab kit, prototype or market-ready product

Anti-drug antibody assays

Development, transfer and valiation of ADA and neutralizing antibody assays for species-independent testing

Anti-drug antibody assays

Development, transfer and valiation of ADA and neutralizing antibody assays for species-independent testing