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Microcoat announces the 2nd Monocyte-Activation Test Proficiency Test Program

Microcoat announces the 2nd Monocyte-Activation Test Proficiency Test Program

MAT Proficiency Test Program

We offer quality assurance to laboratories working with Monocyte Activation Test (MAT). Based on our expertise we provide frequently audits of MAT test proficiency. Our Proficiency Test Program (PTP) confidentially verifies working procedures, method and report validation, identifies trends and potential demands for training. This supplies additional value to internal quality assurance.

 Benefits

  • Check and validate analysis techniques
  • Verify conformity of procedures to requirements of the Pharmacopoeia
  • Control and correct possible drift in procedural compliance
  • Verify laboratory’s ability to recover correct level of contamination
  • Compare results to those of other laboratories and other MAT methods
MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
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Microcoat adds Quanterix´s SP-X to its portfolio of ultra-sensitive Immunoassays

Microcoat adds Quanterix´s SP-X to its portfolio of ultra-sensitive Immunoassays

We proudly announce that we have expanded our ultra-sensitive immunoassay portfolio by the brand-new Quanterix SP-X® Analyzer.

Once again, Microcoat is one of the first laboratories in Europe to offer an advanced new technology to their customers.

The Quanterix SP-X® Imaging and Analysis System is a complete benchtop system that offers true multiplex detection at both acute and baseline levels. Up to 10 parameters can be analyzed with superior sensitivity in this ECL based assay format with a low sample volume of only 25 µL. SP-X combines Simoa ultra-sensitive assay development and optimization with up to 10-plex multiplexing scale and flexibility to combine the analytes of interest.

The SP-X® completes our portfolio of biomarker assays especially in the fields of cytokine quantification, oncology and immune-oncology. Combined with our experience in immunoassay development, the platform enables the development of Simoa-sensitive planar assays meeting your individual requirements.

More than 55 commercial kits available at Quanterix ®.

For more information visit www.quanterix.com.

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
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Meet us at the A3P, Lyon (5. – 6. June 2019)

Meet us at the A3P, Lyon (5. – 6. June 2019)

A3P ENDOTOXINS & PYROGENS, Lyon (5. – 6. June 2019)

Speaker: Dr. Johannes Reich

Title: LPS structure and understanding of LER effect

For more information visit https://en.a3p.org/a3p-endotoxin-pyrogen-lyon-5-6-June-2019/

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE

Technical Report No. 82 „Low Endotoxin Recovery“

Technical Report No. 82 „Low Endotoxin Recovery“

The PDA Technical Report No. 82 has been published in March 2019.

This report gives guidance on how to perform a Low Endotoxin Recovery (LER) study and shows mitigation strategies of endotoxin masking in biologics. Additionally the report is a good source of background information on bacterial endotoxin testing.

At Microcoat we perform LER studies based on the recommendation of the PDA Technical Report 82.

For more information click here: Service https://www.microcoat.de/laboratory-services/endotoxin-and-pyrogen-test-service-2/#Low-Endotoxin-Recovery-LER

Click here for the PDA Technical Report overview

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE

Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

Lonza Endotoxin Summit, Annapolis, USA (3. – 5. June 2019)

Speaker: Dr. Ruth Röder

Title: Mitigation Strategies for Low Endotoxin Recovery

For more information visit http://www.lonza.com/endosummit

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE

Low Endotoxin Recovery – Masking of Naturally Occurring Endotoxin

Low Endotoxin Recovery – Masking of Naturally Occurring Endotoxin

Endotoxins are cell wall components of Gram-negative bacteria. A release of endotoxins into the human blood stream results in an inflammation reaction that can lead to life-threatening conditions like sepsis. Therefore, control for endotoxin contamination of intravenously administered drugs is crucial.

Drugs are usually tested for putative endotoxin contamination with Limulus-based tests. However, validity of the compendial test procedures is questioned in the case of low endotoxin recovery (LER).

To assure validity, regulatory authorities request hold-time studies of endotoxin in addition to pharmacopoeial requirements. Within these studies, endotoxin is added (spiked) to an undiluted product. The spiked product is held for a certain period of time and subsequently diluted for endotoxin determination. Due to the known heterogeneity of endotoxin the question has been raised as to which source represents the most adequate endotoxin spike.

In the present study, endotoxin hold-time studies were analyzed by using different sources of endotoxin. Highly purified endotoxin, crude endotoxin extracts (Naturally Occurring Endotoxin) from different bacterial species and varied growth conditions as well as endogenous endotoxin contaminations were investigated.

The results clearly demonstrate that endotoxin masking – an effect of LER – is dependent on the endotoxin source used. Various parameters such as bacterial strain and growth conditions lead to different masking susceptibilities. Due to these effects it is impossible to predict the susceptibility of bacterial endotoxin contamination to LER. In order to determine whether a sample is prone to LER, an endotoxin spike that is susceptible to LER is required.

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE

Meet us at the 14th Annual Biomarkers Congress 2019

Meet us at the 14th Annual Biomarkers Congress 2019

Dr. Patricia Wolf will be presenting a poster about ultra-sensitive biomarker quantification methods at the 14th Annual Biomarkers Congress 2019

21 – 22 February 2019, Manchester, UK

Click here for more informations: 14th Annual Biomarkers Congress 2019, Manchester

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE

The Endotoxin-Service announces the succesful participation on the LAL PTP study B18

The Endotoxin-Service announces the succesful participation on the LAL PTP study B18

The Endotoxin-Service announces the successful participation on the LAL PTP study B18.

With this result we can look back on 5 years of successful LAL PTP studies.

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE

Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

PharmaLab

Düsseldorf/Neuss, Germany

20 – 21. November 2018

https://www.pharmalab-kongress.de/

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE

We now offer affordable ultra-sensitive biomarker detection with Simoa™ technology

We now offer affordable ultra-sensitive biomarker detection with Simoa™ technology

We are proud to announce that we now offer an exciting immunoassay platform to our customers that opens up new horizons in biomarker analysis. As one of the very first in Europe we have introduced the benchtop Simoa™ Quanterix SR-X™ Biomarker Detection System.

Simoa™ is a single molecule digital detection technology that represents a platform for multiplexed detection of up to 6 biomarkers per sample. It provides ultra-sensitive detection capabilities, while it remains just as affordable as a regular ELISA assay. Additionally, low volume requirements conserve precious samples. The SR-X™ traps and isolates individual immunocomplexes on paramagnetic beads in thousands of femtoliter-sized wells on a disposable disc. Only one single molecule is needed to obtain a suitable fluorescence signal.

Not only does Quanterix offer over 70 commercially available Simoa kits, the SR-X™ also enables fast assay development tailored to our customers’ demands for both circulating proteins and nucleic acids from blood, without complex pre-analytical extraction and PCR. Customers can rely on our extensive expertise in the development of bead-based assays.

Thus, no matter whether you desire exploratory routine sample measurements or seek to develop novel assays of great efficiency, we can offer the implementation of a vast variety of large molecule assays on Simoa™ technology. The SR-X™ enables us to detect biomarkers with an unparalleled sensitivity, 1000x higher than standard immunoassay methods with high precision across a range of sample types and according to highest quality standards.

MORE EVENTS
  • 13Mai

    Meet us at the A3P, Lyon (5. – 6. June 2019)

    Read More →
  • 25Apr

    Meet us at the Workshop „Bioindicators“, Barcelona (07. May 2019)

    Read More →
  • 05Apr

    Meet us at the Lonza Endotoxin Summit, (3. – 5. June 2019)

    Read More →
  • 05Apr

    Vortrag auf dem Concept Heidelberg Lehrgang aktuelle Trends bei Endotoxin- und Pyrogentests

    Read More →
  • 22Jan

    Meet us at the 14th Annual Biomarkers Congress 2019

    Read More →
  • 21Sep

    Meet us at the PharmaLab 2018 Düsseldorf/Neuss, Germany

    Read More →
  • 25Mai

    Meet us at the Lonza Endotoxin Summit, (12. – 14. June 2018)

    Read More →
  • 25Mai

    Participation to job exchange at the Hochschule Biberach HBC

    Read More →
  • 27Feb

    Low Endotoxin Recovery/Masking Training Munich/Bernried, Germany

    Read More →
  • 02Nov

    Visit us at our booth at the Munich Biomarker Conference 2016

    Read More →
READ MORE